All you need to know to generated a valuable genetically engineered model MasterClass

Online MasterClass | 04 & 05 November , 2021

In this MasterClass, the different steps that are requiered to generate a pertinent genetically engineered model will be detailed and explained. What is the purpose of a specific design? What does a conditional model mean? Is it better to fuse a reporter in N- or C-term of a protein? Should we use IRES (Internal Ribosome Entry Site) rather than 2A peptides when we need to coexpress two proteins under the same promoter. What sequences are required? The course will be largely illustrated with mouse and rat model examples and also cellular models. Case studies will illustrate the cours. A time for discussion and question will be left to participant. The advantages and inconveniences of using genome edition (focusing on CRISPR/Cas9 approaches) versus standard homologous recombination will largely be discussed.

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EVENT INTRODUCTION

In this MasterClass, the different steps that are requiered to generate a pertinent genetically engineered model will be detailed and explained. What is the purpose of a specific design? What does a conditional model mean? Is it better to fuse a reporter in N- or C-term of a protein? Should we use IRES (Internal Ribosome Entry Site) rather than 2A peptides when we need to coexpress two proteins under the same promoter. What sequences are required? The course will be largely illustrated with mouse and rat model examples and also cellular models. Case studies will illustrate the cours. A time for discussion and question will be left to participant. The advantages and inconveniences of using genome edition (focusing on CRISPR/Cas9 approaches) versus standard homologous recombination will largely be discussed.

Who Should Attend?

• Research Scientist

• Research Engineers

• Project managers

• Veterinarian

• Medical (Pharmaceutical) Leaders

• Senior Scientist

• R&D leader

• R&D scientist

• Biomedical Engineers

• Senior Research Scientist

• Lab heads

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The level of professionalism of the trainer. Fine-tuned program without wasting time

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Clear and interesting way of presenting issues, good examples and very knowledgeable trainer.

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All lectures of the trainer was exactly what I was looking for and it was directly related to my experties and area that I am working. On top of that he prepared all lectures in a very practical way and also took his time to asnwers to the questions and he made sure that we got the asnwer. The organization of this class was also very good.

Pharmaceutical professional

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Comprehensive overview with very knowledgeable and experienced trainers. The break-out sessions were great as they made the course more interactive and allowed us to apply the concepts in practice.

Banking professional from Europe

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